SUMO2/3 polyclonal antibody
产品名称： SUMO2/3 polyclonal antibody
英文名称： SUMO2/3 polyclonal antibody
产品编号： PAB2408
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of SUMO2/3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SUMO2/3.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- SUMO modification of the Ets-related transcription factor ERM inhibits its transcriptional activity.
Degerny C, Monte D, Beaudoin C, Jaffray E, Portois L, Hay RT, de Launoit Y, Baert JL.J Biol Chem. 2005 Jul 1;280(26):24330-8. Epub 2005 Apr 27.
- Applications
- Western Blot (Cell lysate)
- The SUMO2/3 polyclonal antibody (Cat # PAB2408) is used in Western blot to detect SUMO2/3 in HeLa cell lysate.
- Western Blot
- COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels) . Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels : cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels : cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB with SUMO1 polyclonal antibody (Cat # PAB2402) . Bottom panels : cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO2/3 polyclonal antibody (Cat # PAB2408) . (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the SUMO2/3 polyclonal antibody (Cat # PAB2408) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 6612
- Protein Accession#:
- P55854 (Gene ID : 6612);P61956 (Gene ID : 6613)
- Gene Name:
- SUMO3
- Gene Alias:
- SMT3A,SMT3H1,SUMO-3
- Gene Description:
- SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
- Omim ID:
- 602231
- Gene Ontology:
- Hyperlink
- Gene Summary:
- SUMO proteins, such as SUMO3, and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However, unlike ubiquitination, which targets proteins for degradation, sumoylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability (Su and Li, 2002 [PubMed 12383504]).[supplied by OMIM
- Other Designations:
- OTTHUMP00000115275,SMT3 suppressor of mif two 3 homolog 1,small ubiquitin-like modifier protein 3
- Entrez GeneID:
- 6613
- Protein Accession#:
- P55854 (Gene ID : 6612);P61956 (Gene ID : 6613)
- Gene Name:
- SUMO2
- Gene Alias:
- HSMT3,MGC117191,SMT3B,SMT3H2
- Gene Description:
- SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
- Omim ID:
- 603042
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
- Other Designations:
- SMT3 suppressor of mif two 3 homolog 2,sentrin 2,small ubiquitin-like modifier 2, isoform a