FITC标记的起始识别复合蛋白亚基1抗体
产品名称： FITC标记的起始识别复合蛋白亚基1抗体
英文名称： Anti-ORC1L/ORC1/FITC
产品编号： HZ-8998R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-ORC1L/ORC1/FITC Conjugated antibody
FITC标记的起始识别复合蛋白亚基1抗体
| 英文名称 | Anti-ORC1L/ORC1/FITC |
| 中文名称 | FITC标记的起始识别复合蛋白亚基1抗体 |
| 别 名 | HSORC1; MmORC1; orc1; ORC1_HUMAN; ORC1L; Origin Recognition Complex 1; Origin recognition complex subunit 1 (yeast homolog) like; Origin recognition complex subunit 1; Origin recognition complex subunit 1 homolog; Origin recognition complex subunit 1 like (S. cerevisia; Origin recognition complex subunit 1 like; Origin recognition complex subunit 1 S. cerevisiae homolog like; Origin recognition complex, subunit 1 like (yeast); PARC1; Replication control protein 1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 细胞周期蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 97kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ORC1L/ORC1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation (1). Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins (1). ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors (1). Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis (2). ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin (3,4). ORC4 and ORC6 are also expressed constantly throughout the cell cycle (5,6). ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble (7,8). The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells (8). Function: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Subcellular Location: Nucleus. Similarity: Belongs to the ORC1 family. Contains 1 BAH domain. Database links: Entrez Gene: 4998 Human Omim: 601902 Human SwissProt: Q13415 Human Unigene: 17908 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
DNA复制的启动是一个多步骤的过程，它依赖于复制前复合物的形成，这触发了启动（1）。在建立这些复合物所需的蛋白质中，是原产地识别复合物（ORC）蛋白（1）。ORC蛋白与复制源特异性结合，在复制源上它们作为组装额外启动因子(1)的支架。人ORC亚基1-6在增殖细胞和组织的细胞核中表达，如睾丸（2）。ORC1和ORC2在整个细胞周期中均以等效浓度表达，然而，只有ORC2与染色质(3，4)保持稳定结合。ORC4和ORC6在整个细胞周期（5，6）中也不断表达。ORC2、ORC3、ORC4和ORC5形成ORC6和ORC1组装的核心复合物（7，8）。该核心复合物的形成提示ORC蛋白在哺乳动物细胞G1-S转化中起关键作用（8）。