UBA1 polyclonal antibody
产品名称： UBA1 polyclonal antibody
英文名称： UBA1 polyclonal antibody
产品编号： PAB1772
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of UBA1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBA1.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Complementation by a cloned human ubiquitin-activating enzyme E1 of the S-phase-arrested mouse FM3A cell mutant with thermolabile E1.
Ayusawa D, Kaneda S, Itoh Y, Yasuda H, Murakami Y, Sugasawa K, Hanaoka F, Seno T.Cell Struct Funct. 1992 Apr;17(2):113-22.
- 2.
- Human ubiquitin-activating enzyme (E1): compensation for heat-labile mouse E1 and its gene localization on the X chromosome.
Kudo M, Sugasawa K, Hori T, Enomoto T, Hanaoka F, Ui M.Exp Cell Res. 1991 Jan;192(1):110-7.
- 3.
- Molecular cloning, sequence, and tissue distribution of the human ubiquitin-activating enzyme E1.
Handley PM, Mueckler M, Siegel NR, Ciechanover A, Schwartz AL.Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):258-62.
- Applications
- Western Blot (Cell lysate)
- The UBA1 polyclonal antibody (Cat # PAB1772) is used in Western blot to detect UBA1 in HL-60 cell lysate.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with UBA1 polyclonal antibody (Cat # PAB1772) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 7317
- Protein Accession#:
- UBA1_HUMAN
- Gene Name:
- UBA1
- Gene Alias:
- A1S9,A1S9T,A1ST,AMCX1,GXP1,MGC4781,SMAX2,UBA1A,UBE1,UBE1X
- Gene Description:
- ubiquitin-like modifier activating enzyme 1
- Omim ID:
- 314370
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq
- Other Designations:
- A1S9T and BN75 temperature sensitivity complementing,OTTHUMP00000023197,OTTHUMP00000023198,UBA1, ubiquitin-activating enzyme E1 homolog A,ubiquitin-activating enzyme E1
- Gene Pathway
- Related Disease