FITC标记的脱帽酶1A抗体
产品名称： FITC标记的脱帽酶1A抗体
英文名称： Anti-DCP1A/FITC
产品编号： HZ-10788R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-DCP1A/FITC Conjugated antibody
FITC标记的脱帽酶1A抗体
| 英文名称 | Anti-DCP1A/FITC |
| 中文名称 | FITC标记的脱帽酶1A抗体 |
| 别 名 | DCP1 decapping enzyme homolog A; Dcp1a; DCP1A_HUMAN; mRNA decapping enzyme 1A; mRNA-decapping enzyme 1A; Smad4 interacting transcriptional co activator; Smad4-interacting transcriptional co-activator; Smad4-interacting transcriptional co-activator; SMAD4IP1; SMIF; Transcription factor SMIF. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 染色质和核信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DCP1A |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Cleavage of the 5'-cap structure is involved in the major 5'-to-3' and nonsense-mediated mRNA decay pathways. The protein complex consisting of Dcp1 and Dcp2 has been identified as the species responsible for the decapping reaction in Saccharomyces cerevisiae. In nonsense-mediated decay, the human decapping complex, made up of S. cerevisiae homologs hDcp1a and hDcp2, may be recruited to mRNAs containing premature termination codons by nonsense-mediated decay factor (Upf) proteins. hDcp2 specifically hydrolyzes methylated capped RNA to release m(7)GDP, thereby aiding in mRNA degradation. Both hDcp1a and hDcp2 colocalize in the cytoplasm. In addition, hDcp1a interacts with Smad4 forming a complex with TGF Beta and BMP-4. hDcp1a and Smad4 interact directly through a EVH1/WH1 domain on hDcp1a and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of hDcp1a as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of hDcp1a eliminates TGF Beta-induced nuclear translocation of hDcp1a. Function: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. Subunit: Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Binds DCP1B, UPF1 and SMAD4. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with PNRC2. Interacts with DDX17 in an RNA-independent manner. Interacts with ZC3HAV1. Subcellular Location: Cytoplasm, P-body. Nucleus. Co-localizes with NANOS3 in the processing bodies. Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4. Tissue Specificity: Detected in heart, brain, placenta, lung, skeletal muscle, liver, kidney and pancreas. Similarity: Belongs to the DCP1 family. Database links: Entrez Gene: 55802 Human Entrez Gene: 75901 Mouse Entrez Gene: 361109 Rat Omim: 607010 Human SwissProt: Q9NPI6 Human SwissProt: Q91YD3 Mouse Unigene: 476353 Human Unigene: 28733 Mouse Unigene: 206852 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
5’-CAP结构的裂解参与了主要的5’-3’和无义介导的mRNA衰变途径。由Dcp1和Dcp2组成的蛋白复合物已经被鉴定为酿酒酵母中负责解链反应的物种。在无义介导的衰变中，由酿酒酵母同源物hDcp1a和hDcp2组成的人脱落复合物可被无义介导的衰变因子（Upf）蛋白募集到含有过早终止密码子的mRNA中。HDCP2特异性水解甲基化封端RNA以释放M（7）GDP，从而帮助mRNA降解。HDCP1A和HDCP2在细胞质中共定位。此外，HDCP1A与Smad4相互作用，形成与TGF-β和BMP-4的复合物。HDCP1A和Smad4通过HDCP1A上的EVH1/WH1结构域和Smad4上富含脯氨酸的激活结构域直接相互作用。Smad4对hDcp1a的核转运至关重要，因为hDcp1a的Smard4相互作用结构域（位于N端100个氨基酸）的缺失消除了TGFβ诱导的hDcp1a的核转运。