分子量:20.4 kDa

纯度:>90% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human HVEM, Strep Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA.
Immobilized Human HVEM, Strep Tag (Cat # HVM-H5283) at 2 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag (Cat # BTA-H5255) with a linear range of 0.01  0.5 μg/mL.

产品特性:Human HVEM, Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 20.4 kDa. The predicted N-terminus is Leu 39. The reducing (R) protein migrates as 27-50 kDa in SDS-PAGE  due to glycosylation.

产品背景:Herpesvirus entry mediator (HVEM) is also known as TNFRSF14, TR2 (TNF receptorlike molecule) and ATAR (another TRAF associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily.  HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D (gD). Two TNF superfamily ligands, including the secreted TNFβ (lymphotoxin α) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM.  Besides HVEM, LIGHT can also interact with LTβR, the receptor for lymphotoxin αβ heterotrimer. The role of the HVEM LIGHT /LTβ receptor ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.